Lysine—tRNA(Pyl) ligase

lysine-tRNA(Pyl) ligase

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metabolic pathway

PDB structures

RCSB PDB PDBe PDBsum

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In enzymology, a lysine-tRNAPyl ligase (EC 6.1.1.25) is an enzyme that catalyzes the chemical reaction

ATP + L-lysine + tRNAPyl $\rightleftharpoons$ AMP + diphosphate + L-lysyl-tRNAPyl

The 3 substrates of this enzyme are ATP, L-lysine, and tRNA(Pyl), whereas its 3 products are AMP, diphosphate, and L-lysyl-tRNA(Pyl).

This enzyme belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. The systematic name of this enzyme class is L-lysine:tRNAPyl ligase (AMP-forming).

References

Srinivasan G, James CM, Krzycki JA (2002). "Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA". Science. 296 (5572): 1459–62. doi:10.1126/science.1069588. PMID 12029131.
Hao B, Gong W, Ferguson TK, James CM, Krzycki JA, Chan MK (2002). "A new UAG-encoded residue in the structure of a methanogen methyltransferase". Science. 296 (5572): 1462–6. doi:10.1126/science.1069556. PMID 12029132.

Enzymes: CO CS and CN ligases (EC 6.1-6.3)

6.1: Carbon-Oxygen	Aminoacyl tRNA synthetase (Tyrosine, Tryptophan, Threonine, Leucine, Isoleucine, Lysine, Alanine, Valine, Methionine, Serine, Aspartate, D-alanine-poly(phosphoribitol) ligase, Glycine, Proline, Cysteine, Glutamate, Glutamine, Arginine, Phenylalanine, Histidine, Asparagine, Aspartate, Glutamate, Lysine)

6.2: Carbon-Sulfur	Succinyl coenzyme A synthetase - Acetyl Co-A synthetase - Long fatty acyl CoA synthetase

6.3: Carbon-Nitrogen	Glutamine synthetase - Ubiquitin ligase (Cullin, Von Hippel-Lindau tumor suppressor, UBE3A, Mdm2, Anaphase-promoting complex, UBR1) - Glutathione synthetase - CTP synthase - Adenylosuccinate synthase - Argininosuccinate synthetase - Holocarboxylase synthetase - GMP synthase - Asparagine synthetase - Carbamoyl phosphate synthetase (I, II) - Gamma-glutamylcysteine synthetase

B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, 4.1/2/3/4/5/6, 5.1/2/3/4/99, 6.1-3/4/5-6

Ligases: carbon-carbon ligases (EC 6.4)

Biotin dependent carboxylase	Pyruvate carboxylase · Acetyl-CoA carboxylase · Propionyl-CoA carboxylase · Methylcrotonyl-CoA carboxylase

Other	Polyketide synthase

B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, 4.1/2/3/4/5/6, 5.1/2/3/4/99, 6.1-3/4/5-6

Enzymes: Phosphoric ester and nitrogen-metal ligases (EC 6.5-6.6)

6.5: Phosphoric Ester	DNA ligase

6.6: Nitrogen-Metal	Magnesium chelatase - Cobaltochelatase

B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, 4.1/2/3/4/5/6, 5.1/2/3/4/99, 6.1-3/4/5-6